The nuclear pellet was resuspended in high salt extraction buffer containing 25% v?v?1 glycerol and KCl 0
The nuclear pellet was resuspended in high salt extraction buffer containing 25% v?v?1 glycerol and KCl 0.5?M, as well as the nuclear extract was obtained by pelleting for 30?min in 105,000in an Optima TL ultracentrifuge (Beckmann) utilizing a TLA 100.2 rotor. 4?mM whereas aspirin and salicylate produced just 36C43% inhibition at the same focus. Inhibition…