The reaction was amplified by avidin-biotin-alkaline phosphatase system (ABC kit, PK-4000; Vector Laboratories, Inc
The reaction was amplified by avidin-biotin-alkaline phosphatase system (ABC kit, PK-4000; Vector Laboratories, Inc., Burlingame, CA, USA) and developed with fast red-naphthol (Sigma). higher levels of pro-inflammatory cytokines and IgG2a subclass antibodies. Vertical transmission resulting from chronic infection of immunocompetent is considered a rare event, being attributed instead to either reactivation or reinfection. That is,…