A control for the response was a buffer containing 0
A control for the response was a buffer containing 0.1 M phosphate at pH 7.4 in your final level of 50 l. KCl, 1 MgCl2, 1 CaCl2, and 10 HEPES (pH 7.4) for the shower option and 150 KCl, 5 NaCl, 1 MgCl2, 10 HEPES, and 0.1 EGTA (pH 7.4) for the pipette option (Faria…