After 48 h, cells were knockdown and harvested analyzed by American Blot
After 48 h, cells were knockdown and harvested analyzed by American Blot. Immunoprecipitation, In-Solution and In-Gel Digestions Cells were lysed in IP lysis buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 5 mM EDTA, 1% TritonX-100) containing protease and Evocalcet phosphatase inhibitor cocktail (Sigma). that underlie Compact disc8+ T cell replies to bacterias in…